The relevance of the study was determined by the insufficient study of the lymph changes in various pathological conditions of cows and the lack of effective approaches to lymphatic effusions diagnosis and treatment in veterinary practice. Thus, the aim of this scientific study was to improve the diagnosis of lymphatic effusions in cows and methods of their treatment. Clinical, planometric, cytological, haematological and statistical research methods were used in the study. The feasibility of using an environmentally friendly, natural remedy – a solution of Poltava bischofite – was substantiated. Clinical studies diagnosed similar lymphatic effusions in the area of the cow’s withers, caused by injuries sustained due to design flaws in the farm’s equipment. The development of lymphatic effusions was characterised by the formation of a weak inflammatory reaction, as evidenced by a relatively low number of leukocytes in the puncture. In smears from the lymph puncture, an increase in the number of dystrophically altered epithelial cells and leukocyte accumulation was noted. The therapy involved performing a relieving puncture, after which a 10.0% iodine solution (traditional method) was injected into the effusion cavity of some animals, and a solution of Poltava bischofite (proposed method) was injected into others. The clinical condition of the cows was monitored and the composition of the lymph effusion puncture was determined. A temporary exacerbation of the inflammatory reaction was noted after the introduction of the Poltava bischofite solution into the cavity, which was accompanied by an increase in the number of leukocytes and total protein content in the effusion puncture. During the treatment of cows with the Poltava bischofite solution, a decrease in the number of leukocytes in the blood was recorded, indicating its stimulating effect on the restoration of affected tissues. The number of erythrocytes and haemoglobin content in the blood of these cows also increased. The proposed method of therapy using Poltava bischofite solution has shown high therapeutic efficacy and can therefore be recommended as a therapeutic agent for traumatic effusions in cows. The results of the scientific study have practical value for veterinary doctors who ensure animal welfare
cattle; animal welfare; closed mechanical injuries; lymph; Poltava bischofite
[1] Alitalo, K. (2011). The lymphatic vasculature in disease. Nature Medicine, 17(11), 1371-1380. doi: 10.1038/nm.2545.
[2] Alonso, F.H., Behling-Kelly, E., & Borjesson, D.L. (2022). Lipoprotein profile of pleural and peritoneal transudates in dogs and cats. Journal of Veterinary Internal Medicine, 36(2), 464-472.doi: 10.1111/jvim.16369.
[3] ARRIVE. (n.d.). ARRIVE guidelines. Retrieved from https://arriveguidelines.org.
[4] Arroz-Madeira, S., Bekkhus, T., Ulvmar, M.H., & Petrova, T.V. (2023). Lessons of vascular specialization from secondary lymphoid organ lymphatic endothelial Cells. Circulation Research, 132(9), 1203-1225. doi: 10.1161/CIRCRESAHA.123.322136.
[5] Barbosa, N.V., da Silva, R.S., Engelmann, A.M., Bonamigo, R., Mello, C.B.E., & de Andrade, C.M. (2024). Lactate dehydrogenase activity and total protein as diagnostic markers for cavitary effusions and identification of neoplastic effusions in dogs and cats. Ciencia Rural, 54(11), article number e20230332. doi: 10.1590/0103-8478cr20230332.
[6] Center, S.A. (2012). 10 – fluid accumulation disorders. In M.D. Willard & H. Tvedten (Eds.), Small animal clinical diagnosis by laboratory methods (pp. 226-259). Amsterdam: Elsevier. doi: 10.1016/B978-1-4377-0657-4.00010-7.
[7] Directive No. 2010/63/EU of the European Parliament and of the Council “On the Protection of Animals Used for Scientific Purposes”. (2010, September). Retrieved from https://eur-lex. europa.eu/eli/dir/2010/63/oj/eng.
[8] Du Preez, K. (2023). Effusion fluid analysis: Transudates and exudates. Retrieved from https:// www.sava.co.za/vetnews/2016/2023%20April/VN%20CPD%20April%202023.pdf.
[9] European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes. (1986, March). Retrieved from https://rm.coe.int/168007a67b.
[10] Febo, E., Del Signore, F., Bernabò, N., Paolini, A., Simeoni, F., De Bonis, A., Rosto, M., Canal, S., & Vignoli, M. (2023). Ultrasonography and sonoelastography characteristics of benign vs. malignant mesenteric lymph nodes in cats: An update. Animals, 13(16), article number 2664. doi: 10.3390/ani13162664.
[11] Funk, D., & Neiger, R. (2014). Usefulness of lactate dehydrogenase concentration in thoracic and abdominal fluid of dogs. Schweiz Arch Tierheilkd, 156(12), 577-583. doi: 10.1024/00367281/a000653.
[12] Furnaris, F.C., & Constantin, T.N. (2024). Perspective chapter: Exploring multifaceted approaches to enhance dairy cow welfare. London: IntechOpen. doi: 10.5772/intechopen.115031.
[13] Hansen, K.C., D’Alessandro, A., Clement, C.C., & Santambrogio, L. (2015). Lymph formation, composition and circulation: A proteomics perspective. International Immunology, 27(5), 219-227. doi: 10.1093/intimm/dxv012.
[14] Hein, W.R., Shelton, J.N., Simpson-Morgan, M.W., Seamark, R.F., & Morris, B. (1988). Flow and composition of lymph from the ovary and uterus of cows during pregnancy. Journal of Reproduction and Fertility, 83(1), 309-323. doi: 10.1530/jrf.0.0830309.
[15] Janardhan, H.P., Jung, R., & Trivedi, C.M. (2023). Lymphatic system in organ development, function, and regeneration. Circulation Research, 132(9), 1181-1184. doi: 10.1161/ CIRCRESAHA.123.322867.
[16] Jerlström, J., Lind, A., Lindah, C., Berg, C., & Wallenbeck, A. (2025). Traumatic injuries detected at slaughter іn cattle: Impact of production system and season on animal welfare and meat condemnation in Sweden. Acta Veterinaria Scandinavica, 67, article number 18. doi: 10.1186/ s13028-025-00804-x.
[17] Jian, Y., Zhou, J., Pan, W., Chen, J., Zhang, Y., Li, Y., Liu, X., Xiao, S., Deng, C., & Wei, Z. (2025). Decellularized lymph node scaffolds accelerate restoration of lymphatic drainage in rat hind limb lymphedema. Bioengineering & Translational Medicine, 10(6), article number e70056.doi: 10.1002/btm2.70056.
[18] Khol, J.L., Pinedo, P.J., Buergelt, C.D., Neumann, L.M., Baumgartner, W., & Rae, D.O. (2012). The collection of lymphatic fluid from the bovine udder and its use for the detection of Mycobacterium avium subsp. paratuberculosis in the cow. Journal of Veterinary Diagnostic Investigation, 24(1), 23-31. doi: 10.1177/1040638711425943.
[19] Koltowska, K., Jakus, Z., Hong, Y., & Kume, T. (2023). Editorial: Lymphatic system: Organ specific functions in health and disease. Frontiers in Cell and Developmental Biology, 11, article number 1224584. doi: 10.3389/fcell.2023.1224584.
[20] Kyrychko, O.B., Kyrychko, B.P., Sherstyuk, L.M., & Panova, A.M. (2021a). Haematological and biochemical indices of blood in cats with feline panleukopenia when using Poltava bischofite solution. Scientific Progress & Innovations, 4, 233-238. doi: 10.31210/visnyk2021.04.31.
[21] Kyrychko, O.B., Kyrychko, B.P., Titarenko, О.V., & Sydorenko, V.V. (2021b). Poltava bischofite solution application for enteroinfections prevention and calves colostrum immunity formation. Scientific Progress & Innovations, 2, 213-219. doi: 10.31210/ visnyk2021.02.27.
[22] Law of Ukraine No. 3447-IV “On the Protection of Animals from Cruelty”. (2006, February). Retrieved from https://zakon.rada.gov.ua/go/3447-15.
[23] Lee, J.C., & Lee, S.K. (2024). Post-traumatic chylous wrist effusion: A case report. Journal of Hand Surgery Global Online, 6, 430-434. doi: 10.1016/j.jhsg.2024.02.008.
[24] Monnet, E. (2004). Pleural effusions. In World Small Animal Veterinary Association world congress proceedings (article number 3852314). Toronto: WSAVA.
[25] Oblak, M.L., Lu, H.Y., Ram, A.S., & McKenna, C. (2024). Comparative aspects of targeted sentinel lymph node mapping in veterinary and human medicine: Opportunities for future research. Frontiers in Medicine, 11, article number 1342456. doi: 10.3389/fmed.2024.1342456.
[26] Peek, S.F. & Divers, T.J (2018). Rebhun’s diseases of dairy cattle (3rd ed.). St. Loui: Elsevier.
[27] Ponikowska, B., Fudim, M., Iwanek, G., Zymliński, R., & Biegus, J. (2025). Harnessing the lymphatic system. Heart Failure Reviews, 30, 673-683. doi: 10.1007/s10741-024-10449-z.
[28] Quantrill, S.J., & Dabal, L. (2002). Transudates and exudates. Thorax, 57(7). doi: 10.1136/ thorax.57.7.657-a.
[29] Quick, C.M., Criscione, J.C., Kotiya, A., Dongaonkar, R.M., Hardy, J., Wilson, E., Gashev, A.A., Laine, G.A., & Stewart, R.H. (2014). Functional adaptation of bovine mesenteric lymphatic vessels to mesenteric venous hypertension. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 306(12), R901-R907. doi: 10.1152/ajpregu.00185.2013.
[30] Santambrogio, L. (2018). Chapter four – the lymphatic fluid. International Review of Cell and Molecular Biology, 337, 111-133. doi: 10.1016/bs.ircmb.2017.12.002.
[31] Smuts, C.M., Mills, J.N., & Gaál, T. (2016). Transudate or exudate: Сan lactate dehydrogenase activity in canine and feline effusions help to differentiate between the 2? Veterinary Clinical Pathology, 45(4), 525-733. doi: 10.1111/vcp.12397.
[32] Sotillo, S., Viall, A.K., Palerme, J.S., & Ward, J.L. (2025). Retrospective evaluation of the causes and fluid characteristics of cavitary effusions in dogs and cats. Journal of Veterinary Internal Medicine, 39(5), article number e70205. doi: 10.1111/jvim.70205.
[33] Tahara, M., Katsumi, A., Akazawa, T., Otsuka, Y., & Kitahara, S. (2011). Post-traumatic chylous knee effusion. The Knee, 18(2), 133-135. doi: 10.1016/j.knee.2010.02.009.
[34] Weaver, L., & Weaver, B. (2025). Examining the lymphatic system of the ruminant. In G. Leith, S. Eaton, R.E. Englar, S. Bentley & L. Hallam (Eds.), Performing the large animal physical examination (pp. 445-460). Hoboken: John Wiley & Sons. doi: 10.1002/9781119830382.ch19.
[35] Weber, E., Aglianò, M., Bertelli, E., Gabriele, G., Gennaro, P., & Barone, V. (2022). Lymphatic collecting vessels in health and disease: A review of histopathological modifications in lymphedema. Lymphatic Research and Biology, 20(5), 468-477. doi: 10.1089/lrb.2021.0090.