Ukrainian Journal of Veterinary Sciences

The results of studies on determination of chronic toxicity of Mospilan and Akhtara insecticides from the neonicotinoids group, whose active substances are acetamiprid and thiamethoxam, respectively are represented in the paper. The manifestation of chronic toxicity was assessed by changes in the indices of the clinical state, morphological and biochemical indices of blood, pathomorphological studies. Clinical manifestations of poisoning and animals death were not observed. Investigations of the blood morphological composition at chronic poisoning with Mospilane after its introduction in 1/10 DL50 dose, thrombocytosis and neutrophilic leukocytosis including right shift were registered, as well as lymphocytopenia. Thrombocytosis and leukocytosis also developed at poisoning with Akhtara in the appropriate dose, but without changes of the leukogram. In blood serum of animals after Mospilan and Akhtara poisoning the content of the total protein increased due to the globulin fraction. In the serum of white mice poisoned by Mospilan, the urea content was reduced by 43.6% against the constant creatinine content and a significant increase of the activity of ALAT, AspAT, and a simultaneous increase of GTP activity, which indicated significant dysfunction of the synthetic liver function with hepatocytolysis in animals. Increased GTP activity without changes in ALT activity indicated dysfunction in the hepatobiliary system at chronic poisoning with Akhtara. A significant increase of AspAT activity under the influence of Mospilan and Akhtara confirms the effect of both pesticides on nicotine-sensitive receptors in mice. The more significant influence of Mospilan as compared to Akhtara on the blood system and liver is associated with a higher degree of toxicity of the active substance.

neonicotinoids, Mospilan, Akhtara, acetamiprid, thiamethoxam, toxicity, laboratory mice, blood, liver, enzyme activity