The methicillin-resistant Staphylococcus aureus (MRSA) is a global challenge in veterinary medicine due to its responsibility for nasal infection in goats. The present study was carried out to estimate the prevalence of this bacterium in goats and to detect their antimicrobial resistance profiles. A total of 153 nasal swab samples were collected from goats, and a questionnaire was used to collect necessary data related to this study. A standard laboratory analysis was done to recognise MRSA and to determine its antimicrobial sensitivity. Among 153 samples, 20.9% were found positive for Staphylococcus aureus based on results of cultural and biochemical properties. Overall, 4.58% of the samples were identified to be mecA gene positive. The prevalence of MRSA was higher in young goats aged below 1 year at 7.14%. In the case of female goats, the prevalence was 5.05%, which was greater than males at 3.70%. In Black Bengal goats, the prevalence was highest at 11.11%, and in the semi-intensive rearing system, the prevalence was 7.89%. Methicillin-resistant Staphylococcus aureus showed 100% resistance against Cefoxitin and Oxacillin. The organism also showed 42.86% resistance to Ampicillin, Erythromycin, and Oxytetracycline, followed by 14.29% to Ceftriaxone and Gentamicin, and 28.57% to Ciprofloxacin, respectively. The findings of 4.58% MRSA and multidrug-resistant MRSA in goats indicate a significant public and animal health concern. By identifying goats as potential carriers of MRSA, the findings emphasise the need for improved surveillance, biosecurity, and antibiotic stewardship in livestock farming. These results can guide veterinarians, farmers, and policymakers in developing effective strategies to control the spread of resistant bacteria from animals to humans, particularly in regions where close humananimal interactions are common
Prevalence; mecA gene; Antimicrobial Sensitivity Testing (AST); Staphylococcal infection, Risk Factors, Goat
[1] Abdullahi, I.N., Lozano, C., Saidenberg, A.B.S., Latorre-Fernández, J., Zarazaga, M., & Torres, C. (2023). Comparative review of the nasal carriage and genetic characteristics of Staphylococcus aureus in healthy livestock: Insight into zoonotic and anthroponotic clones. Infection, Genetics and Evolution, 109, article number 105408. doi: 10.1016/j. meegid.2023.105408.
[2] Altaf, M. (2019). Molecular characterization of methicillin resistant Staphylococcus aureus (MRSA) and associated risk factors with the occurrence of goat mastitis. Pakistan Veterinary Journal, 40(1). doi: 10.29261/pakvetj/2019.079.
[3] Bangladesh Bureau of Statistics. (2024). Livestock economy at a glance (2023-2024). Retrieved from https://dls.portal.gov.bd/sites/default/files/files/dls.portal.gov.bd/page/ee5f4621_ fa3a_40ac_8bd9_898fb8ee4700/2024-08-13-10-26-93cb11d540e3f853de9848587fa3c81e.pdf.
[4] Bangladesh National Portal. (2022). Bangladesh economic review 2022. Retrieved from https://mof.portal.gov.bd/site/page/28ba57f5-59ff-4426-97ab014242179e/BangladeshEconomic--eview-2022.
[5] Barua, P., Rahman, S.H., & Barua, M. (2021). Sustainable value chain approach for livestockbased livelihood strategies for communities of the southeastern coast of Bangladesh. Modern Supply Chain Research and Applications, 3(3), 191-225. doi: 10.1108/MSCRA-08-2020-0021.
[6] Broekema, N.M., Van, T.T., Monson, T.A., Marshall, S.A., & Warshauer, D.M. (2009). Comparison of cefoxitin and oxacillin disk diffusion methods for detection of mecA-mediated resistance in Staphylococcus aureus in a large-scale study. Journal of Clinical Microbiology, 47(1), 217-219. doi: 10.1128/jcm.01506-08.
[7] Clinical and Laboratory Standards Institute. (2020). Performance standards for antimicrobial susceptibility testing (30th ed.). Wayne: CLSI.
[8] Egyir, B., Hadjirin, N. F., Gupta, S., Owusu, F., Agbodzi, B., Adogla-Bessa, T., Kwasi Addo, K., Stegger, M., Larsen, A.R., & Holmes, M.A. (2020). Whole-genome sequence profiling of antibiotic-resistant Staphylococcus aureus isolates from livestock and farm attendants in Ghana. Journal of Global Antimicrobial Resistance, 22, 527-532. doi: 10.1016/j.jgar.2020.03.029.
[9] European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes. (1986, March). Retrieved from https://rm.coe.int/168007a67b.
[10] FAO. (2018). Shaping the future of livestock: Sustainably, responsibly, efficiently. Retrieved from https://openknowledge.fao.org/items/050a596e-c861-4405-ba8f-bc5246b6a2ac.
[11] Gharsa, H., Slama, K.B., Lozano, C., Gómez-Sanz, E., Klibi, N., Sallem, R.B., Gómez, P., Zarazaga, M., Boudabous, A., & Torres, C. (2012). Prevalence, antibiotic resistance, virulence traits and genetic lineages of Staphylococcus aureus in healthy sheep in Tunisia. Veterinary Microbiology, 156(3-4), 367-373. doi: 10.1016/j.vetmic.2011.11.009.
[12] Haque, M., Islam, M.A., Bhuiyan, A.K.F.H., Akter, A., & Hossain, M.M. (2023). Livelihood improvement of poor farmers through goat rearing. Journal of Agriculture, Food and Environment (JAFE), 4(1), 5-10. doi: 10.47440/JAFE.2023.4102.
[13] Herawati, O, Bejo, S.K., Zakaria, Z., & Ramanoon, S.Z. (2023). The global profile of antibiotic resistance in bacteria isolated from goats and sheep: A systematic review. Veterinary World, 16(5), 977-986. doi: 10.14202/vetworld.2023.977-986.
[14] Islam, M.A., Biswas, P., Sabuj, A.A.M., Haque, Z.F., Saha, C.K., Alam, M.M., Rahman, M.T., & Saha, S. (2019). Microbial load in bio-slurry from different biogas plants in Bangladesh. Journal of Advanced Veterinary and Animal Research, 6(3), 376-383. doi: 10.5455/javar. 2019.f357.
[15] Larsen, A.R., Stegger, M., & Sørum, M. (2008). spa typing directly from a mecA, spa, and pvl multiplex PCR assay – lance. Clinical Microbiology and Infection, 14(6), 611-614. doi: 10.1111/j.1469-0691.2008.01995.x.
[16] Li, X., Robinson, S.M., Gupta, A., Saha, K., Jiang, Z., Moyano, D.F., Sahar, A., Riley, M.A., & Rotello, V.M. (2014). Functional gold nanoparticles as potent antimicrobial agents against multi-drug-resistant bacteria. ACS Nano, 8(10), 10682-10686. doi: 10.1021/nn5042625.
[17] Mechesso, A.F., et al. (2021). Resistance profiling and molecular characterization of Staphylococcus aureus isolated from goats in Korea. International Journal of Food Microbiology, 336, article number 108901. doi: 10.1016/j.ijfoodmicro.2020.108901.
[18] Moroz, A., et al., (2020). Nasal carriage of various staphylococcal species in small ruminant lentivirus-infected asymptomatic goats. Polish Journal of Veterinary Sciences, 23(2), 203-209. doi: 10.24425/pjvs.2020.133634.
[19] Muwonge, K.M., Ndagire, H., Mulindwa, J., & Twesigye, C.K. (2025). Multiple antimicrobial resistance indices of Staphylococcus aureus from the nares of goats and slaughterhouse attendants in Kampala city, Uganda – a cross sectional study. BMC Microbiol, 25, article number 162. doi: 10.1186/s12866-025-03891-y.
[20] Nsofor, C.A., & Patience, O.O. (2019). Prevalence and antibiogram of Staphylococcus aureus isolated from herd of goat, cow and ram at Obinze, Imo State, Nigeria. Microbiology & Infectious Diseases, 3(2), 1-5.
[21] Omoshaba, E.O., Ojo, O.E., Oyekunle, M.A., Sonibare, A.O., & Adebayo, A.O. (2020). Methicillin-resistant Staphylococcus aureus (MRSA) isolated from raw milk and nasal swabs of small ruminants in Abeokuta, Nigeria. Tropical Animal Health and Production, 52(5), 2599-2608. doi: 10.1007/s11250-020-02301-x.
[22] Pal, M., Kerorsa, G. B., Marami, L. M., & Kandi, V. (2020). Epidemiology, pathogenicity, animal infections, antibiotic resistance, public health significance, and economic impact of Staphylococcus aureus: A comprehensive review. American Journal of Public Health Research, 8(1), 14-21. doi: 10.12691/ajphr-8-1-3.
[23] Piva, S., et al. (2021). Epidemiologic case investigation on the zoonotic transmission of infection from goat to veterinarians. Zoonoses and Public Health, 68(6), 684-690. doi: 10.1111/ zph.12836.
[24] Rahimi, H., Saei, H.D., & Ahmadi, M. (2015). Nasal carriage of Staphylococcus aureus: Frequency and antibiotic resistance in healthy ruminants. Jundishapur Journal of Microbiology, 8(10), article number e22413. doi: 10.5812/jjm.22413.
[25] Sakr, A., Brégeon, F., Mège, J.L., Rolain, J.M., & Blin, O. (2018). Staphylococcus aureus nasal colonization: An update on mechanisms, epidemiology, risk factors, and subsequent infections. Frontiers in Microbiology, 9, article number 2419. doi: 10.3389/fmicb.2018.02419.
[26] Titouche, Y., et al. (2024). Phenotypic and genotypic characterization of Staphylococcus aureus isolated from nasal samples of healthy dairy goats in Algeria. Pathogens, 13(5), article number 408. doi: 10.3390/pathogens13050408.
[27] Vitale, M., Galluzzo, P., Buffa, P.G., Carlino, E., Spezia, O., & Alduina, R. (2019). Comparison of antibiotic resistance profile and biofilm production of Staphylococcus aureus isolates derived from human specimens and animal-derived samples. Antibiotics, 8(3), article number 97. doi: 10.3390/antibiotics8030097.